打开谷瀑APP浏览
推广采购上谷瀑环保
立即打开
  • 产品
  • 详情
OPAL 光学氧气测量系统-74001
价格电议,您可以向供应商询价得到该产品价格
上海闵行区
上海净信实业发展有限公司
9年 | 指数:190 | 工商已认证
店铺推荐
  • 图文详情
  • 产品参数

 

货号

产品名称

规格(个/盒)

74001

ibidi OPAL 光学氧气测量系统

1

74051

氧气测量系统适配器

1

11920

ibidi CO2气体控制系统

1

11922

ibidi CO2O2气体控制系统

1

11929

ibidi 气体压力泵

1

 

Applications

1.Exact oxygen measurement in cell cultures or tissue

2.Ideal for in vitro hypoxia conditions, like 3D cultures, spheroid models, and tissue


In addition to controlling the oxygen concentration in the stage top incubator, it is indispensable to know the real oxygen concentration near the cells (monitoring extracellular O2), or even inside the cells (intracellular O2). Because cells consume oxygen, the concentrations are typically much lower in cell clusters, such as tissue or spheroids.

With the ibidi OPAL Optical O2 Measurement Sytem, the oxygen concentration can be measured optically and directly in a 2D cell culture dish, a compact 3D cell spheroid, or even in a piece of tissue. Oxygen-sensitive beads or cell-permeable fluorophores are utilized to measure the fluorescence lifetime.


Note: The OPAL System measures lifetimes in the range of 1-1,000 µsec (i.e., phosphorescence). To keep things simple, ibidi uses the more common term fluorescence, instead of phosphorescence.

Working Principle and Components of the ibidi OPAL System

The ibidi OPAL System is easily connected to your fluorescence microscope.

Compatibility to Microscopes

The ibidi OPAL System can be connected to all modern inverted microscopes from Nikon, Olympus, Leica, and Zeiss. Small adaptations need to be made, and during this process, ibidi will comprehensively support you. These adaptations include a specialized OPAL Filter Set and an LED Light Source Coupler for the microscope (included in the ibidi OPAL System). The OPAL Detector Unit fits the standard C-Mount camera port, or can also be mounted on an ocular.

The selected objective lens defines the measured area. An iris is used to reduce the field of measurement. The density of the CPOx beads or NanO2 labeled cells can be selected to further specify the sample area.

Extracellular O2 Monitoring

 

Oxygen monitoring is defined as the measurements of O2 in the direct neighborhood of cultured cells. Oxygen sensitive beads, with a diameter of 50 µm (CPOx), are used in combination with the ibidi OPAL system or a FLIM microscope to measure the oxygen concentration.

Intracellular O2 Measurement

For intracellular oxygen measurements, an oxygen-sensitive nanoparticle reagent is brought into the cells. The ibidi OPAL System or FLIM microscopes reveal quantitative O2concentrations by identifying a change in the lifetime of the fluorophore NanO2.

Overview: O2 Monitoring and O2 Imaging

品牌:德国ibidi
加工定制:
型号:74051 11920 11922 11929
规格型号:74051 11920 11922 11929
内容声明:谷瀑环保为第三方平台及互联网信息服务提供者,谷瀑环保(含网站、客户端等)所展示的商品/服务的标题、价格、详情等信息内容系由店铺经营者发布,其真实性、准确性和合法性均由店铺经营者负责。谷瀑环保提醒您购买商品/服务前注意谨慎核实,如您对商品/服务的标题、价格、详情等任何信息有任何疑问的,请在购买前通过谷瀑环保与店铺经营者沟通确认;谷瀑环保设备网上存在海量店铺,如您发现店铺内有任何违法/侵权信息,请在谷瀑环保PC版首页底栏投诉通道进行投诉。